FLEX2 Basic utilizes Nova’s groundbreaking MicroSensor Card™ technology to provide a fully automated cell culture analyzer that eliminates biosensor maintenance, increases analyzer speed, and reduces sample volume. The full 11-test cell culture menu includes: Gluc, Lac, Gln, Glu, NH4+, Na+, K+, Ca++, pH, PCO2, PO2
Simple touchscreen operation, a choice of sampling modes, fast analysis time, and automated quality control (QC) provide simplicity, labor savings, and workflow efficiency for cell culture optimization and monitoring.
Chemistry and gas sensors are combined in the credit-card-sized MicroSensor cards, which use proven Nova biosensor technology that has been validated in thousands of cell culture processes. Cards are maintenance free, have a minimum use life of 21 days, and are replaceable in seconds.
Onboard liquid QC provides true verification of performance and saves hours of labor each week compared to manual QC testing. QC cartridges contain up to a 30-day supply of QC material. Controls are run automatically at user-selected intervals.
Glucose, lactate, glutamine, glutamate, ammonium, sodium, potassium, and calcium measurements are performed using electrochemical biosensors integrated into one credit-card-sized component.
Broad analytical range with improved low end accuracy
The lower analytical range has been extended to provide accurate results down to 0.10 g/L for glucose/lactate and 0.10 mmol/L for glutamine/glutamate. An external load-and-go sample tray allows for continuous loading of cell culture samples for the most flexible and efficient sample throughput.
Robotic automation and a precise metering syringe perform all dilutions on board, eliminating time-intensive manual dilutions and error associated with manual techniques. The use of onboard auto-dilutions provides the broadest analytical range of any cell culture analyzer.
Chemistries are unaffected by cell concentration
Photometric detection methods used by other analyzers often require time-consuming, manual pre-dilution of high cell concentration samples to avoid poor measurement accuracy. FLEX2 Basic provides accurate results regardless of cell concentration.
Nova’s biosensors are developed specifically for cell culture applications, where processes typically utilize extremely complex media formulations. These electrochemistry detection methods provide excellent specificity for the analyte of interest.
High throughput chemistry analysis
FLEX2 Basic is capable of analyzing a full chemistry panel using 135 µL of sample in 120 seconds, or a throughput of 30 samples per hour. Full 96-well plates can be analyzed in about three hours.
pH, PCO2, and PO2 measurements are performed by potentiometric and amperometric biosensors. Biosensors are located in the maintenance-free, disposable MicroSensor Card.
Unlike hospital blood gas analyzers, which are often used for cell culture analysis, Nova’s algorithms for measuring pH, PCO2, and PO2 were specifically developed for cell culture applications. These algorithms are optimized for mammalian cultures, which typically exhibit much higher oxygen consumption and carbon dioxide production rates compared to human blood cells. FLEX2 Basic ensures accurate pH and gas values even in cultures with high cell densities.
Gas and pH values available in manual and 96-well plate analysis modes
The user can configure FLEX2 Basic to provide gas and pH results from both manual samples and 96-well microtiter plate samples.
Electronic record retention and retrieval
FLEX2 Basic meets GMP manufacturing requirements through installation qualification (IQ) and operational qualification (OQ) documentation and validation support from Nova specialists.
The purchase of a FLEX2 Basic analyzer is just the beginning of a long commitment from Nova to you. Nova offers an extensive array of support services to help maintain peak analyzer performance.
We maintain a highly skilled and experienced technical support staff available by phone or email. From basic questions to advanced troubleshooting, they are able to resolve most problems over the phone without requiring on-site support.
Sample Analysis Time: 2.0 minutes (Chemistry only), 2.0 minutes (Gas only), 2.0 minutes (All tests)
Operating Temperature Range:10–30oC (50–86oF)
Operating Relative Humidity Range: 20–85%
Sample Size: 135 to 265 µL
Sample Options: Individual via syringe/cup
Automated batch using 20-position tray or 96-well plates
Operating System: Windows 10
Electrical Requirements: 90-264 VAC, 50 to 60 Hz (Universal Power Supply)
System Size: Height: 24 in (61 cm), Width: 17 in (43 cm), Depth: 25 in (64 cm)
System Weight: 94 lb (42.6 kg) without reagent packs
Safety Certifications: IEC 61010-1:2001, Quality Systems Certification: ISO 9001:2008
OPC Compliant, PAT Compatible, 21 CFR Part 11 Compliant